Description
Make your target specific gRNA lentivectors with desired markers!pLenti-gRNA-U6 / H1- Cloning Kit is designed for cloning of double-strand DNA encoding your desired gRNA sequence for CRISPR genomic gene editing. Once transcribed, the target specific gRNA will guild CAS9 enzyme to cut the genomic DNA at desired loci. To make your target specific gRNA lentivector, two synthetic gRNA oligonucleotides are first annealed to form the double stand DNA duplex which is then cloned into the provided, linear pLenti-gRNA-U6 / H1 lentivector via T4 enzyme ligation.
