Description
Established from a small squamous cell carcinoma of the tongue of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat-PCR analysis the Y chromosome could not be detected in this cell line when tested at Glow Biologics. It cell line is a known phenomenon that SSC cell lines can lose their Y chromosome.
Adherent Suspension
Adherent
Suggested Medium
DMEMHAMS F12 (11) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Quality Control
H103 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
H103 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
H103 Cell Line is for research use only
