Description
The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).
Adherent Suspension
Suspension
Suggested Medium
Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)
Quality Control
GM1899A Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Lymphoblastoid
Shippment
GM1899A Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
GM1899A Cell Line is for research use only
