Description
The SV40 transformed breast epithelial cell line fR2 was obtained in 1982 by infecting suspensions of primary milk cultures with wild-type SV40. The cells have been shown to express Muc-1, also known as HMFG-1 antigen, which cell line is a mucin-like component of human milk fat globule membranes. They are positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumorigenic in nude mice.
Adherent Suspension
Adherent
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS
Quality Control
fR2 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial, cuboidal
Shippment
fR2 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
fR2 Cell Line is for research use only
