Description
B9 cell line is a mouse B cell hybridoma line that requires Interleukin 6 (IL-6) for survival and proliferation. IL-6 cell line is a pluripotent cytokine with multiple effects on many different cell types. It is produced by a variety of cells in response to various stimuli. The B9 hybridoma allows for a simple sensitive assay for human, rat, rabbit, pig and mouse IL-6 based on the IL-6 dependent proliferation of this murine B cell line. Details of the B9 hybridoma growth factor assay can be found in Current protocols in Immunology 1996 Cytokines and Their Cellular receptors 6.6.1-6.6.5 contributed by Nordan RP, Richards CD and Gauldie J. John Wiley & Sons, Inc.
Adherent Suspension
Suspension
Suggested Medium
IDMEM or RPMI 1640 + 2mM Glutamine + 50µM 2-Mercaptoethanol (2ME) + 50pg/ml recombinant human IL-6 (Sigma SRP3096, diluted and stored in PBS containing 1% BSA) + 5% FBS. IL-6 needs to be added fresh at each media change.
Quality Control
B9 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Lymphoblastoid
Shippment
B9 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
B9 Cell Line is for research use only
