Description
The retrovirus packaging Ampli-GPE (clone A57) cells were obtained from NIH3T3 fibroblasts being co-transfected with two kinds of recombinant chimeric proviral genes in the BMG Neo vector. To reduce the homology between proviral DNA in the packaging cell and the retroviral vector, the gag-pol and env genes of Moloney murine leukaemia virus were separated onto two different plasmids in which the 5' long terminal repeat (LTR) and the 3' LTR had been replaced by the mouse metallothionein I promoter or the rabbit ß-globin gene. This expression and amplification system produces a viral titre high enough for gene transfer to somatic cells (up to 5x1,000,000 CFU/ml) and avoids the generation of replication-competent viruses.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 250µg/ml G418 + 10% Foetal Bovine Serum (FBS).
Quality Control
Ampli-GPE Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
Ampli-GPE Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Ampli-GPE Cell Line is for research use only
